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In this review, we summarize the major advances in TALE technology over the past several years with a focus on the interaction between TALEs and DNA, TALE design and construction, potential applications for this technology, and unique characteristics that make TALEs superior to zinc finger endonucleases.
To facilitate better understanding of the functional relevance and mechanisms regulating genome architecture, we applied transcription activator-like effector (TALE) technology to visualize endogenous repetitive genomic sequences in mouse cells.
The presence of methylation on DNA target regions was initially recognized as a potential limitation of the TALE technology.
Transciption activator-like effector (TALE) technology has recently emerged as an alternative robust and efficient genome editing tool [ 3, 4].
The simplified TALE assembly combined with multiple choices of vectors will facilitate the broad use of TALE technology.
The choices of a number of useful vector sets should further broaden TALE technology to various applications.
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CEO of Professional Science Editing for Scientists @ prosciediting.com