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Docked conformation of the most active molecule in the series, which shows desirable interactions in the receptor, was taken as template for alignment of the molecules.
The licenses (see Figure8a) which are searched from Internet are taken as template objects.
For cDNA synthesis, random hexamer primers were used (Thermo Scientific, USA) and 1 µg of the DNase treated RNA sample was taken as template.
Here, the full length HA of the Influenza A H1N1/1918 virus [23] was taken as template for the estimation of the most probable structure of protein HA63 286-RBD.
After centrifugation, 2 µl of the supernatant was taken as template DNA and added to a 25 µl reaction mixture containing 0.1 µl of each primer pair in a 10 pmol concentration, 0.3 µl 10 mM of the four deoxynucleoside triphosphates (Sigma-Aldrich Chemie GmbH, Munich Germany), 2.5 µl of 10x PCR buffer, 1 µl of 50 mM magnesium chloride and 1 unit of Taq-Polymerase (Rapidozym GmbH, Berlin Germany).
Fifty ng genomic DNA derived from primary tumor tissue was taken as template.
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These short fragments were taken as templates for first-strand cDNA synthesis using random hexamer-primer.
137– 144 In template-based modeling, one or several 3D structures of protein showing a reasonable degree of similarity to the protein of interest are taken as templates.
These short fragments were taken as templates to synthesize the first-strand cDNA using hexamer primers and superscript™III (Invitrogen™, Carlsbad, CA, USA).
Plant DNA samples were amplified with the Illustra Genomi Phi V2 DNA Amplification Kit (GE Healthcare Europe GmbH, Munich, Germany) and 250 ng each of the resulting reaction products were taken as templates for genotyping.
After that, the model with optimum shape and period parameters is taken as a template to approximate the impulses in faulty bearing signal.
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