Because the vanadium tailings (VT) obtained from vanadium extraction are regarded as waste material with a low reuse rate, the recycling of VT is environmentally desirable and economically feasible.
The kits for DNA purification, gel recovery, plasmid miniprep and A-Tailing modification were obtained from Tiangen (Beijing, China) or Takara (Dalian, China).
Three combinations were performed: (i) the small RNA fraction with the long RNA fraction, both of which were directly labeled by 3'-tailing, (ii) labeled cDNAs obtained from both RNA fractions, and (iii) a combination of directly labeled small RNA fraction and labeled cDNA derived from the long RNA fraction.
The screening experiment showed that 45 strains with the NH4 +-N-degrading ability were obtained from the tailings of REE mines.
Surface waters (0 10 cm) were obtained from Suncor tailings ponds and from MLSB, as well as from Syncrude's West In-Pit pond (Table S2), as described elsewhere.
The solid content of MFT obtained from oil sands tailings was adjusted to 1, 3, 5 wt % with water before applying 15, 30, and 60 min of ozonation.
pELMO digestion with the SmaI enzyme produced a blunt-ended vector; this bypassed the A-tailing reaction step for PCR fragments obtained by high fidelity polymerases.
The tailing factors of AR and impurities obtained on the Agilent TC-18 column were between 0.8 and 1.2, which indicated good peak shapes for all compounds.
The profiles are easily obtained for both active and decommissioned tailings facilities because their data can be collected through field investigations.
The optimum parameters to prepare the permeable bricks were obtained at 1180 1200 °C for 45 min with 20 wt% tailings, 60 70 wt% gangue and 10 20 wt% waste ceramic.
