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The stress-induced hyperalgesia was measured by tail withdrawal latency.
In Tail immersion test, both the extracts showed significant increase in tail withdrawal response (p < 0.001).
In vivo, in the mouse tail withdrawal assay, UFP-112 (1 100 pmol, i.c.v).
Meanwhile, the analgesic activity of the same compounds was evaluated using the rat tail withdrawal technique.
T treatment did not affect basal nociception on either the hotplate or tail withdrawal tests, but significantly increased morphine's antinociceptive potency on the tail withdrawal test — however, this effect was small, and independent of duration of T exposure.
Spontaneous hyperalgesia was observed in the tail withdrawal assay following chronic morphine in C57BL/6J, but not 129P3/J mice.
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It has been reported that N/OFQ can block antinociceptive effects induced by opioid receptor agonists in the radiant heat tail-flick test and warm water tail-withdrawal test.
Briefly, a beam of light was focused on the dorsal surface of the mouse tail and the time until the tail flicked was monitored (tail-withdrawal latency) [ 32].
The significant increase (p < 0.05) in tail-withdrawal time by the extract suggests the involvement of central mechanisms in its antinociceptive effect.
The latency period of the tail-withdrawal response was taken as the index of antinociception and was determined at 30, 60, 90, and 120 min after the treatments.
This test is based on the observation that morphine like drugs selectively prolongs the reaction time of the typical tail-withdrawal reflex in mice [ 20, 21].
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