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Specifically, lumen formation of intersegmental vessels was markedly reduced, leading to visibly reduced blood flow in the trunk and tail (data not shown).
These mice were not significantly longer that the WT mice as determined by measurement from nose to tail (data not shown).
In control tissue (rat epididymal sperm), the antibody labelled caudal to the middle-piece/principal piece boundary of the sperm tail (data not shown), as has been described previously [21].
Although we obtained reproducible binding of ezrin to the two longest peptides, we did not observe any interaction between ezrin and our truncated peptide (figure 9C) or a control peptide containing amino acids 53 73 of the Podocalyxin cytoplasmic tail (data not shown).
NUDE mice (in opposition to their nu/+ littermates) showed similar clinical signs of infection to Rag1−/− mice, including decreased activities, disseminated lesions in the tail (data not shown) and significant weight loss (p<0.001, unpaired t test, Figure 6A), culminating with the death of all animals in the group (Mean survival: 17 days, p<0.001 compared to nu/+ animals, Figure 6B).
In contrast, similarly targeting U2 snRNP did not affect the activity of a branch site-containing tail (data not shown).
Similar(50)
Shapiro-Wilkes tests and Quantile-Quantile analysis demonstrated that expression was approximately log-normally distributed for most targets, with slight deviations in the tails (data not shown).
Injection of Rasl11b* had minor effects on wild-type embryos, only occasionally reducing the tail tip (data not shown).
At 1 week of age, chimeric pups were identified by performing genomic PCR of the eGFP transgene from DNA of tail biopsies (data not shown).
The histogram of shRNA abundance showed a heavy tail distribution (data not shown), therefore we took a log transform to stabilize the variation for better power in later statistical analysis.
To verify the sensitivity and reliability of the comet assay in detecting DNA damage we conducted tests that showed that treatment of cells by peroxide or MMS produced a significant increase in the tail moment (data not shown).
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