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Subsequently, the PCR-based screening approach resulted in the amplification of a 1,245 bp-long cDNA fragment, termed cF6H, that contained the 3'-end of this putative cDNA clone, including an in-frame stop codon (TAA), a putative polyadenylation signal (ATATAA) and a short polyA tail (Accession # AY605048), although it lacked the translation start site.
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Both ferret HEV genomes consisted of 6,820 nt and a poly (A) tail (GenBank accession nos. AB890001 and AB890374), and nucleotide sequence identity was 99.6%.
The genome of Em2007 was 15,272 bp, including the poly(A) tail (GenBank accession no. EU262603), and shared 87.6% and 57.9% sequence identity with VR-2332 and LV, respectively, indicating that Em2007 belongs to the North American genotype.
The complete nucleotide (nt) sequences from CRMD reference trees 95CI192R3 and B48-C consist of 8,397 and 8,398 nt, respectively, excluding the poly A-tail (GenBank accessions KC218926 and KC218927, respectively).
The mRNA encoding the splice variant with the short cytoplasmic tail CEACAM1-4S (GenBaccessionsionono.
The sequence of the 3′ end of open reading frame (ORF 1, the full-length ORF2, ORF3, and the noncoding region through the poly-A tail was determined (GenBank accession no. EF450827).
The genome was 30,117 nt long, including 12 nt at the 3′ poly A tail (MERS-CoV camel/Qatar_2_2014, GenBank accession no. KJ650098).
Paired-end RNA-seq data from Latimeria chalumnae tissues, including the gills, kidneys, pectoral fins, pelvic fins, pharynx and tail muscle, were obtained from SRA accession DRP000627.
There are five publicly available sequences for swine WC1 including one unique Domain 1 sequence and two unique intracytoplasmic tail sequences (swppWC1 and swWC1-29e1; GenBaccessionsionumbersrs CAA67710 and CAA67709, respectively).
The V-105 genome consisted of 6,927 nt plus a poly (A) tail of a still-undetermined length (GenBank accession no. JX120573).
The HelitronFinder program identified two adjacent, head-to-tail Helitrons in a maize BAC sequence with GenBank accession number AF466202 (Fig. 7).
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