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The benzyl-type fluorous tags were prepared in 3 steps from a commercially available fluorous alcohol.
For genotyping, RAD tags were prepared from genomic DNA from background parental strains N32 and N2977, as well as mutant and wild-type progeny pools from the N32 and AX7 cross.
Sequence tags were prepared by the Leiden Genome Technology Center (Leiden, the Netherlands) as described [ 30].
Sequence tags were prepared with Illumina's Digital Gene Expression Tag Profiling Kit, according to the manufacturer's protocol.
To obtain gene expression profiles, sequencing libraries containing 21-nt tags were prepared from 6 μg total RNA (for developmental stage-specific expression profiles) or 2 μg total RNA (for cell type-specific expression profiles) using the Illumina Gene Expression Sample Prep Kit (Illumina, San Diego, CA, USA).
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When Au nanorod-based SERS tags are prepared as we have described previously, the degree of aggregation, as evidenced by a red-shift in the extinction spectrum, can be affected by the acid washing step used to remove CTAB from the rod surface.
Two recombinant proteins containing the poly-histidine N-terminal tag were prepared using the plasmid pQE30 (Qiagen Inc).
Baculoviruses expressing these mutants with C-terminal His tag were prepared in the same way as wild-type MERS-CoV RBD.
The trace tag was prepared by loading signal antibodies and high-content glucose oxidase on amino-functionalized silica nanosphere.
In the present study, a form of ubiquitin having the C-terminus modified with the addition of a biotinylation peptide tag was prepared.
The array was prepared by immobilizing captured antibodies on ultralong Ag nanowires, whilst the trace tag was prepared by loading horseradish peroxidase (HRP -labeled goat anti-HRP -labeledRP-anti-Igoaton thionine (TH)-doped mesoporous ZnO nanostrawberries (MP-ZnO).
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