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The libraries were filtered on several levels to leave only useful tags for analysis.
Twenty-one tags were removed as they contained ambiguous (N) nucleotides leaving 48,905 tags and 22,907 unique long SAGE tags for analysis.
To generate tags for analysis, we used a filtering procedure involving the DiscoverySpace 4.0 application (http://www.bcgsc.ca/platform/bioinfo/software/ds) (filtering details, see Methods) [ 44].
LongSAGE libraries were sequenced to 310,072 - 339,864 tags each, with a combined total of 2,931,124 tags, and filtered to leave only useful tags for analysis (Table 1).
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A spokesman says: "In the limited number of cases where tamper alerts have been raised, and where we have had access to the tag for analysis, we have been able to prove either definite evidence of deliberate interference, or in one case, evidence of damage which would be consistent with falling or catching the device on a hard surface.
A list of the tags used for analysis was provided in Table 1 and detailed information about these libraries are available from the GEO website.
The germline SAGE library identified 92,007 tags, which was normalized to 100,000 tags for further analysis.
All identical Solexa reads in each library were sorted into unique sequence tags for further analysis.
This yielded more than 2 million high-quality tags for further analysis.> -wrap-foot> tagsthat that started with a stretch of Ts.
The ability to acquire fully human monoclonal antibodies (mAbs) with pre-defined specificities is critical to the development of molecular tags for the analysis of receptor function in addition to promising immunotherapeutics.
A number of haplotypes were found to be associated with increased risk of breast cancer, all of which could be attributed to a single marker (intron 24: rs206340) that was not selected as a tag SNP for analysis in our study.
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