Sentence examples for tagging the expression from inspiring English sources

SNPs tagging the expression haplotypes were correlated with the total expression of several genes in breast tissue measured by Taqman and microarray technologies.

The minor allele of SNP rs4942440, tagging the high expression haplotype 4, was associated with a reduced breast cancer risk (per-allele HR = 0.85, 95% C.I. = 0.72 to 1.00, P-trend = 0.048).

For examples, a florescence reporter can be introduced to tag the protein expression and the effects of MiMIC insertions can be reverted after excising the whole cassettes.

The reason for the difference is that epitope tagging influences the expression of this receptor and complex-glycosylated GLP-1Rs are largely reduced after epitope tagging at the N-terminus.

To enable the remove of LSL-tag, tobacco etch virus (TEV) protease recognition sequence was placed downstream of LSL-tag in the expression vector, and LSL-tagged TEV protease, designated LSL-TEV, was also expressed in E. coli., and was recovered with purity of 82 ± 5%, and yield of 85±2%2% from crude extract of recombinant E. coli.

These tags restrict the expression of tagged proteins to specific phases of the cell-cycle, due to control elements of cyclin Clb6 or Clb2, respectively (Karras & Jentsch, 2010; Hombauer et al, 2011).

Here we demonstrate its application and effectiveness as an N-terminal fusion tag for the expression and purification of proteins that could not be effectively produced with other tags.

In all cases, the presence of the CPD-His6 fusion tag increased the expression and solubility of target proteins.

Immunoblot analysis using antibodies against epitope tags confirmed the expression of these membrane proteins in the BV fractions (Fig. 1).

It is feasible for the practical use of MoaD as a fusion tag in the expression of some target proteins.

The presence of the GFP-tag in the expression vectors used enabled us to establish the fraction of C2C12 cells that were actually transfected.