To tag the cells, one of three MCF7-Ctrl (B1) and MCF7-Six1 (A13) clones was transduced with pLNCX2-ZsGreen retrovirus and selected by fluorescence activated cell sorting (FACS).
Using these datasets, the entire C. elegans neuronal network becomes a colored graph where nodes represent neurons, edges are connections between neurons, and the color of the node tags the cell-type of the node.
However, they were not tagging the ovarian cancer cells labeled with trastuzumab or anti-HER-2 biosimilars.
The anti-HBsAg from the vaccinated patients were only tagging the ovarian cancer cells labeled with AVEC: anti-HER-2 × HBsAg.
To test this hypothesis, the researchers tagged the reprogrammed cells, called induced pluripotent stem (iPS) cells, with a fluorescent dye and injected them into early-stage mouse embryos.
Two weeks after the injury, the scientists tagged the crushed cells with fluorescent markers to assess cell growth and survival.
In order to detect antibodies to Caspr2, we expressed full-length human Caspr2 in HEK293 cells, tagging the protein by introducing EGFP at the intracellular C-terminus.
NH2-lipid-SPMNP seems to tag all the cells within 60 min of SPMNP-cell interaction, while DMSA-SPMNP and PEG-lipid-SPMNP shows 70 and 40% magnetic cell tagging respectively (Fig. 4c).
When DU145 cells were transiently overexpressed with dn-WOX1 (EGFP tag), the cells resisted C1q-induced apoptosis (Fig. 3).
Where indicated cells were co-transfected with expression plasmids for a hyperactive mutant of ERO1 (C133A, C133A; ERO1*) tagged by the cell-surface marker CD2 and wild-type or inactive mutants of ChaC1 fused to mCherry.
