Called Safe-Sequencing System (Safe-SeqS), the strategy entails tagging individual template molecules with unique identifiers (or barcodes), which are then amplified and read out by next generation DNA sequencing.
HTML's New Template Tag: Standardizing Client-Side Templating Eric Bidelman discusses the new HTML tag template.
The box tag template for the exterior of the box (you'll need a hole punch too).
We examined the sensitivity to partial alkaline hydrolysis (Figure 7) of peptides designed to incorporate methionine between two His6 tags (template f).
The explanation lies in the fact that different tag template sequences were used in these assays.
Likewise, the tag template after the resume codon can be altered with little or no effect [ 12].
The four pseudoknots in tmRNA can be replaced with unrelated sequences and structures as can the tag template [ 11, 25].
The ssrA tag template sequence encodes the last 14 amino acids of the kanamycin resistance protein (KanR), ANKLQFHLMLDEFF, instead of the normal degradation tag, ANDENYALAA.
The plasmids that express tmRNA mutants for the frame misreading assays are based on pCH201, a derivative of pKW11 with a tmRNA tag template encoding ANDH6D [ 18].
