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These targeting vectors, collectively referred to as the Berghei Adaptable Transfection (pBAT) plasmids, contain key elements that permit recycling of the drug-selectable cassette, robust green fluorescent labelling of recombinant parasites, carboxy-terminal tagging of target proteins with a red fluorescent epitope tag fusion, and expression of heterologous genes.
- Tagging of target proteins for tag-based pull downs may interfere with endogenous protein function.
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In addition, more tags of target transcripts sliced by PN-miR1509, PN-miR393 and PN-miR403 which were up-regulated under Al stress were detected in the Al-free library (Additional file 3, Table 4).
Sequenced tags of targets between the two libraries were compared, and the result showed that there were more specific targets detected in the CT library than in the NT library.
To circumvent this problem, tagged versions of target proteins, expressed in relevant cells of interest, are sometimes used.
Nevertheless, the weight of the evidence suggests that they belong to the vast assemblage of ligases catalyzing formation of peptide bonds or similar linkages in the biosynthesis of a variety of compounds and also in the peptide-tag-modification of target proteins.
The intracellular expression of these affinity fluorescent probes in a range of cell types may permit the directed tagging of intracellular targets with fluorescent proteins for a range of molecular cell biology imaging studies currently not possible via direct fluorescent protein fusions.
A meta-analysis of the pairwise signature correlations then computed the correlation of the brown fat signature with the tags of all target signatures (Figure 5A).
Reporter ions originating from the isobaric tags of the target population are indistinguishable from reporter ions originating from any interfering ions.
The LocAtioN iDentification based on dynaMic Active Rfid Calibration LANDMARCC) [22] system uses the k-NN algorithm with reference tags to estimate the locations of target tags.
After applying a criterion to remove 1- or 2-read tags, a considerable fraction of erroneous tags remained, and the number of tags exceeded the number of target molecules estimated from the amount of input DNA.
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