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Thus, more and more approaches/pipelines were developed to recover complete mitochondrial genomes directly from whole-genome shotgun (WGS) sequencing data (Botero-Castro et al. 2013; Hahn et al. 2013; Lavrov et al. 2013; Williams et al. 2014; Li et al. 2015) using reference-independent assembly strategies and species-specific tagging of samples.
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We traced birth holdings by comparing flock marks from identity tags of sampled sheep with flock marks recorded against sheep movements in the Animal Movements Licensing System (AMLS2) database in the period January 2005 to February 2006 inclusive.
Chemical isotope tagging of FFPE samples has been performed using iTRAQ labeling [ 12– 15].
In addition, the transposase-based fragmentation and tagging of the samples sequenced on the Illumina MiSeq disfavors coverage of free ends, making de novo assembly at these ends more difficult.
Gene expression was analyzed by digital gene expression tag profiling of samples taken from P. patens protonema at 3, 14 and 24 days, and from leafy shoot tissues at 30 days, after protoplast isolation, and from 14-day-old caulonemal and chloronemal tissues.
"Mixed fragments" are sequence reads containing a sample identification tag of one sample at one end and the sample identification tag of another sample at the other end (Fig. 5).
This generated more than 4.8 million tags of two samples after sequencing.
The combination of sequence tags at each end of the overlapped reads allowed us to use a small number of primers to uniquely tag a large number of samples.
Alternatively, samples can be labelled using differential mass-tags, which enables groups of samples to be combined and analysed equivalently.
One is pooling of tagged samples with each individual tagged by a unique short barcode.
In sample s1 (Arabidopsis), 8 tags matched Oryza sativa genes, while no Arabidopsis tags were found in data of samples c, k, q or e, suggesting that contamination due to errors in the index sequences indeed occurs, albeit at a low level (data not shown).
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