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Sibert et al. [12] hypothesized recently that bluefin tuna migrations and inter-annual shifts noted with satellite tagging datasets may in part be linked to shifts in oceanographic conditions.
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Analysis of the Fab tagged datasets reveals classes of particles with stalks protruding at distinct angles from the motor.
For subunit localization, reference-free 2D class averages from each tagged dataset were generated.
For the subunit and domain localization, reference-free 2D class averages were generated as described above for each tagged dataset and the native dataset.
The presence of many taxonomically unsolved sequences in the 16S rRNA gene tag datasets from these hot springs could be a sign of novel microbe richness in these less known hot water bodies of Northeastern India.
These tag sequences were composed of the DGE-tag datasets presented in Table 3.
In the counting tags datasets, we assessed the statistical significance of the differences in specialization.
The rRNA reference databases were screened for taxonomically wrongly assigned reference sequences using simulated ribo-tag datasets.
While vertical activity differed across the four tag datasets, the average and maximum daily diving depth values were similar while offshore.
Simulated SSU ribo-tag datasets were aligned to the LSUrdb and vice versa, to estimate the potential of "cross-contamination", i.e. the assignment of SSU rRNA-derived ribo-tags as LSU rRNA ribo-tags and vice versa.
Values for these three tag datasets were significantly different than for 18M (Kruskal-Wallis one way analysis of variance on ranks p = <0.01), although the difference in depths is small.
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