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In conclusion, PIT tagging causes a major disruption to the bacterial community associated with the skin of frogs, as well as a concurrent proliferation in resident fungi.
We find that PIT tagging causes a major disruption to the bacterial community associated with the skin of frogs (∼12-fold increase in abundance), as well as a concurrent proliferation in resident fungi (up to ∼200-fold increase).
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As with GFP, IC tagging caused relocation of Luc to muNS-related inclusions in insect cells co-expressing GFP-muNS-Mi (Figure 5A, panel 4), while untagged Luc showed no association with inclusions (Figure 5A, panel 3).
The game of tag causes near-nuclear destruction in Townsville, tearing holes in skyscrapers and leveling city blocks with fire.
Failure to correctly remove the tag causes the bottle to break.
So we do have an extra bit of pride seeing Yammer's big exit confirmed today (and Steve Ballmer's limo parked outside), even if the price tag causes some to balk just a bit (and complain even more about Yammer's lack of an auto-refresh feature. Grumble grumble).
Neither tag causes nuclear localisation in trypanosomes [11,16].
Again, this relationship is not true for the C terminus, because the TAP tag causes all proteins to have the same C terminus (p = 0.2, Kruskal-Wallis test; Figures S1E and S1F).
Given that the molecular consequences of TAg oncogene expression are well understood, together with our finding that TAg causes the predictable changes in the MexTAg tumours, the model can be reliably used experimentally to represent human disease, keeping TAg effects in mind.
The behavior of reverse transcriptase at the cross-linked nucleotide is also exploited by iCLIP, which takes advantage of the fact that often the amino acid tag causes termination of reverse transcription at that site to determine the precise location of the termination (and thus the cross-linking) site [ 79].
Saints players expressed shock at the suspensions — including a Twitter message from running back Pierre Thomas that said, "we might as well start playing two-hand tag cause this is madness!!" — but reaction around the league was mixed.
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