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The markers developed will be helpful in saturating Lens genetic maps and for tagging and mapping of genes and quantitative trait loci associated with important traits to be further used in marker-assisted breeding for enhancing productivity and quality.
Hamwieh et al. (2005) developed 35 simple sequence repeat (SSR) markers, but these have reported no amplification or limited polymorphism, creating a major bottleneck to gene tagging and mapping studies in this crop.
Collaborative, multidisciplinary research to enhance the development of plant genome resources and information that would be publicly available for gene expression, gene tagging, and mapping has been a priority in recent years in plants of agronomic importance, including soybean [ 6].
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This integrated genetic linkage map will facilitate exploitation of the B. rapa annotated genomic resources for gene tagging and map-based cloning in B. napus, and for comparative analysis of the A genome within Brassica species.
During the last two decades DNA based molecular markers have been extensively used for evaluation of population diversity estimation, germplasm characterization, linkage and QTL analysis, gene tagging, and map-based cloning.
When the birds returned to the breeding grounds the following summer, Shaffer and his team recovered 19 tags and mapped the enormous migratory route of the shearwaters.
In each plot all I. deltoidea plants were tagged with numbered plastic tags and mapped in an X - Y coordinate system.
To analyse the partial and alternative transcripts, the contigs were computationally fragmented to 100 bp tagged and mapped to the banana genome using the bowtie2 programme [ 50].
The distribution of the iCLIP-tags to RNA was analysed (as a percentage of the total number of tags) and mapped to open reading frames, intergenic and intronic regions, non-coding RNA (ncRNA), and untranslated regions (UTRs) (Fig. 1b).
Contigs were trimmed in length from the 5' end, to a minimum length of 20 nt, 36 nt and 50 nt to use as sequence tags and mapped against the annotated ncRNAs.
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CEO of Professional Science Editing for Scientists @ prosciediting.com