Sentence examples for tagged line from inspiring English sources
Since tagged line 17 is hemizygous for the tags, the presence of PCR products with the same size as in the untransformed plant indicates amplification of the wild-type gene copies in tagged line 17.
To find out which sequence(s) activated the luc+ reporter gene in tagged line 17, an RT-PCR approach was followed.
These activity patterns confirmed LUC screening results of the original tagged line 17 as well as the aforementioned RT-PCR results.
This level of induction at 8°C is lower than in the original tagged line 17 (10.7-fold) and might be caused by stability of the GUS enzyme (half-life of more than 60 h).
Although usually different tagged sequences were obtained with the two walking methods, identical sequences were also retrieved in several lines (data not shown), including tagged line 17 (Table 2).
Seeds of the T-DNA tagged line osrecql4-1 and of the Tos17 insertion line osrecql4-2 were obtained from POSTECH (line No. 3A-03503) [ 50] and NIAS collections (line No. NC2763), respectively [ 51].
All mentioned tagged lines are available upon request.
The spot concludes with the "Click instantly" tag line.
