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Multiple isobaric tag samples were normalized by comparing the median protein ratios for the reference channel.
To explore the possibility that there could be variation within the RAD tag samples in sex chromosome divergence that corresponds with the three mitochondrial DNA haplotype groups detailed earlier, we explored nucleotide diversity in male and female individuals from each group.
Mitochondrial sequences from two other samples used in RAD tag sequencing (1 female and 1 male) and seven samples used in PCR screens (4 females and 3 males) were identical to each other and differed from the golden strain mitochondrial sequence by a different single-nucleotide substitution than the previously mentioned sequence present in five of the RAD tag samples.
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Isobaric tag sample was normalized by comparing the median protein ratios for the reference channel.
After digestion with thrombin to remove the 6H tag, sample was again loaded onto the Ni-NTA agarose column.
Two independent groups have established χ2 as the best statistical test to use in tag sampling experiments [ 16, 17].
Previous studies have shown a limited concordance between tag sampling methods and microarrays (van Ruissen et al. 2005; Haverty et al. 2004; Kavsan et al. 2007).
The tag sampled sound with 16-bit resolution at 500 kHz/channel and was synchronized with the array hydrophones (Wisniewska et al., 2012).
The Affymetrix technology that were used to generate the expression measurements has been validated and match results obtained with tag sampling for sufficiently abundant genes [ 45, 46].
This technique, also known as tag sampling or RNA-seq, is considered to be an efficient method for gene expression analysis [ 88, 89].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com