Two purification steps using double-tag properties enabled the separation of fusion proteins including the ability to control the separation from degradation products containing single tags.
Yahoo, in addition to launching a flurry of new products in the last few months (and the pace seems to be accelerating), now owns the two most important tagging properties on the web – flickr and del.icio.us.icio.us
Techcrunch wrote "Yahoo, in addition to launching a flurry of new products in the last few months (and the pace seems to be accelerating), now owns the two most important tagging properties on the web – flickr and del.icio.us".icio.us
The SNPs analyzed in this study have been selected based on previous associations and tagging properties, and are mostly contained within NPSR1 intronic sequences.
The SNPs were selected from dbSNP based on availability and their informativeness as of at the time the study was begun (information regarding tagging properties were limited at the time of SNP selection), with a preference for validated markers with a minor allele frequency (MAF) of >0.2.
For this purpose, 19 single nucleotide polymorphisms (SNPs), spanning the entire 220 kb region of the NPSR1 gene, were selected based on reported associations and/or their tagging properties, and genotyped in 1808 RA cases and 888 healthy controls from the Epidemiological Investigation of Rheumatoid Arthritis (EIRA) study.
There were no alternative SNPs with similar tagging properties.
These are conservative estimates since additional SNPs with undetermined tagging properties were randomly selected from dbSNP.
Assays in which the genotypes of duplicate samples did not show >95% concordance were discarded and replaced with alternative assays with the same tagging properties.
Assays in which the genotypes of duplicate samples did not show greater than 95% concordance were discarded and replaced with alternative assays with the same tagging properties.
