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Thus, the solubility of the tagged protein is most likely influenced by the tag position and by the host strain chosen for the expression.
The two possible target tag position is near.
Significantly, tag position #2 results in the longest average read range of 416.8 cm, and tag position #1 results in the shortest average read range of 221.0 cm.
Comparing the measured tag position and true value of points, the accuracy is validated.
The purpose of experiment is to check the ranging data and tag position accuracy.
Point P is the common point in two triangles; therefore P is the right target tag position.
Similar(29)
In contrast, no effect of the His6-tag position was observed for the production of FtsL, ClfB and IsdB (data not shown for FtsL and IsdB).
For both Classic and Signature MPSS data there is a positive correlation between measured abundance and tag-position for low-range tag-position genes.
We initially suspected that some of the trend observed between tag-position and measured abundance might relate to a correlation between tag-position and these features, eg. high tag-position is more likely for genes with long transcripts.
However, the reason that both Classic and Signature data have a tendency towards increasing measured abundance with tag-position in the low tag-position range is unclear.
However, comparison with the tag-position effect illustrated in Fig 1 shows that tag-position bias is a much more significant effect than these two features.
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