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The desired end product should only contain the fluorescent protein or tag of choice fused to the gene of interest, so after selection of the rare recombination event the selectable marker must be removed.
The reporter tag of choice is introduced in the form of a cassette also containing the selectable marker galK flanked by FRT sites, which will later be targeted by the Flp recombinase (the FRT-galK-FRT module or FgF)(Fig. 3 & Table 1).
Furthermore, for the expression of chimeric versions of MADS-box genes it is favorable to use the entire genomic region in frame with the tag of choice.
Furthermore, for the expression of chimeric versions of MADS-box genes it is favorable to use the entire genomic region in frame to the tag of choice.
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Any pIII or pVIII display phagemid encoding single clones or a library can be used with one of the pVII modified helper phages described, and bispecific display with a pVII-tag of choice is obtained after standard phagemid rescue.
When posting your Instagram photo, include a tag of your choice, and it will post to Twitter as a native image with a card.
Volvo, which often gets tagged as the vehicle of choice for college professors and other well-off liberal professionals, skews Republican in Michigan.
The engineering of appropriate tags into the proteins of choice yields "molecular handles" through which multi-component complexes can be immunoprecipitated and highly purified.
They are easily modified to include tags or reporter molecules of choice.
Expressed Sequence Tags (EST) are tools of choice to investigate the expressed portion of an uncharacterized genome.
For the rest of the fiscal year the mammoth retailer and bastion of bargain-basement wares is going beyond its price tag undercutting for a different weapon of choice against more upscale rival Target : trendier products.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com