Based on Internet of Things (IoT) technology, the sensor tags which are semi-passive tags integrated with sensors can monitor temperature in smart cold chain system.
Mutant libraries with tags integrated into the genome have proved to be a very important tool in yeast research and, recently, a TagModule system based on this tool was developed as a template for tagging the mutants of other organisms [ 108].
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With the aim of quantifying a difference in tag losses between the conventional and the Betyp tags, we integrated the tag type into the tag-attrition model used to fit the recoveries per unit of time.
We hypothesise that this resulted from the tag integrating as an array and one or more copies being lost in progressive rounds of telomere truncation.
The variation in telomere tag signal intensities within a single metaphase spread, in clones that have more than one tagged telomere, indicates that at least some telomeres contain more than one copy of the tag; this is consistent with the possibility that the tag has integrated as an array.
The YFP tag is integrated between aa 3 and 4 of TrxR1, preceding the sites required for the TrxR1 catalytic activity.
Additionally, a 9myc tag was integrated at the C-terminus.
For this analysis, an HA-tag was integrated into the endogenous HMT1 locus in cells also expressing Myc-tagged Ccr4-Not subunits from their endogenous loci.
