Exact(29)
To assess whether internal tagging of proteins within annotated protein domains is more disruptive than tagging within other regions of proteins, we mapped the tag insertion site in each of 200 tagged proteins and determined its position with respect to annotated protein domains.
The total antibody half-life for each aldehyde-tagged ADC was the same as, or longer than, trastuzumab, suggesting that aldehyde tag insertion and HIPS conjugation did not change the basic PK properties of the antibody.
The tag insertion will be applicable to the other fluorescent proteins.
For tag insertion, we made a small incision (2 cm) in the abdominal cavity.
The tag insertion was also successful in other fluorescent protein, mCherry.
To reduce structural stresses resulting from epitope tag insertion the tags were separated from retained urease sequences by a flexible amino acid linker.
Similar(31)
To evaluate possible detrimental effects of the HA epitope tag insertions on protein function, transport properties of individual Slc11a2 tagged proteins were assessed in corresponding LLC-PK1 transfected cells.
We developed the FindInsertSeq workflow to localise the DNA tag insertions to the nucleotide level.
Additionally, certain HA tag insertions causing loss of transport can provide additional clues on structure−function relationships.
We do not observe significant differences between sense oligonucleotides, double- and single-stranded circular DNA, so for ease of experimental design, we recommend oligonucleotides as templates for nucleotide changes and short tag insertions.
On the other hand, no topology data could be obtained for Slc11a2 protein segments delineated by the TM1−2, TM2−3, and TM3−4 intervals and represented by HA tag insertions in constructs 1−3.
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