Sentence examples for tag insert from inspiring English sources

Unlike the previous study, in which long sequences of AtGCS1 were deleted or exchanged mainly [11], we used a different strategy that partly disrupts characteristic AtGCS1 domains with a fluorescence tag insert so that we could see the importance of each domain, and normal expression of the modified AtGCS1s as well.

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One tag inserted into a dusky rockfish failed immediately after insertion and was therefore not monitored.

Using this strategy combined with cryo-EM 3D reconstruction, we were able to visualize the characteristic NZ-1 Fab density attached to the PA tag inserted into a surface-exposed loop in the middle of the sequence of CCT6 subunit present in the Saccharomyces cerevisiae group II chaperonin TRiC/CCT.

A 19 bp foreign sequence tag inserted into the rRNA gene sequence at +92 (Fig. 2A) allowed us to discriminate transgene transcripts from endogenous rRNA transcripts (Fig. 2B).

The human H2B histone tag, inserted into the pEGFP-N1 cloning vector (Clontech), was a generous gift from Dr. Geoffrey Wahl [36].

Importantly, the VSV-G tag was only reactive when inserted into the KE; no reactivity was observed in cells expressing Env with the VSV-G tag inserted into the LLP2 domain.

Recombinant baculovirus was constructed to express human IDE with a hemaglutinin (HA) tag inserted after the second methionine (amino acid 42) of IDE driven by polyhedrin promoter [3], [11] (Fig. 1A).

To this end, we used a HEK293-derived cell line stably expressing ERFAD with an HA tag inserted immediately prior to the C-terminal KEEL sequence (A11 cells, [15]).

Complementation with a single copy of melD (without the His6 tag) inserted into the chromosome of HY69 also restored its catechol resistance to the wild-type level (data not shown).