Sentence examples for tag incorporated from inspiring English sources

PCR is then performed using a transcript-specific forward primer and a reverse primer that is identical to the unique tag incorporated at RT.

A construct to express Dictyostelium cyclin C with an N-terminal FLAG tag under the actin 15 promoter (pDXA[act15::FLAG-cycC]) was generated by amplifying the coding region of CycC from the ATG to the stop codon by PCR from genomic DNA, with the FLAG tag incorporated into the N-terminal primer.

C3-containing isoforms were visualized using a flag antibody that detects an epitope tag incorporated into the design of the C3-specific primer.

This was achieved by subcloning into the pTTQ18-based qacR clone pSK5676 (7), which has a six-histidine tag incorporated into the C-terminus of the expressed QacR protein.

Therefore, the difference in separating the phosphorylated from the non-phosphorylated species between Phos-tag and regular gels was mainly attributable to the effect of Phos-tag incorporated.

Multiple targets were simultaneously encoded with unique MassCode tags incorporated from labeled primers and probes using processes modified from standard PCR.

After transfer, rabbit antisera directed against either the His-tag incorporated at the N-terminal region of GvpC, or a synthetic GvpC peptide (data not shown), were used in Western blotting analysis.

The Daily Vault's Benny Balneg liked that the song disengaged itself from the album's "blues tag" and incorporated more "contemporary elements" into its sound.

A C-terminal c-myc tag was incorporated (underline); this tag was incidental for H. capsulatum expression in this study, but was used in P. pastoris expression as described below.

During cDNA generation, sequence tags were incorporated prior to pooling cDNAs from different conditions (see Material and Methods for details).

The epitope tags were incorporated to facilitate ELISA detection of the NICD cleavage product using anti-HA antibody, and purification of the fusion protein substrate from E.coli lysates using nickel column affinity chromatography, respectively.