A two-year tag effects study was carried out concurrently to evaluate the post-surgical growth, survival, tag retention, health and swimming ability of acoustically tagged smolts [36].
While the laboratory tag effects study showed that surgically implanted Thompson River coho smolts had 100% survival to the end of the detection period observed in the field [36], there may be unforeseen tag effects that have not yet been identified in the wild [59], [61].
The sources can be classified as either positive bias (false survivals e.g. detections from dead fish or tags not actually present), or negative bias (false mortalities e.g. due to tag effects, fish passing by undetected or fish residualising in the upper watershed).
Complete sequences for all protein constructs, concentration- and light-dependent effects on PT dynamics, NMR saturation transfer for connectivity analysis, fitting and simulation methods for FCS and NMR, E222Q titrations, and local His tag effects.
Given that the molecular consequences of TAg oncogene expression are well understood, together with our finding that TAg causes the predictable changes in the MexTAg tumours, the model can be reliably used experimentally to represent human disease, keeping TAg effects in mind.
Thus, body size of the model species becomes a major limiting factor; yet few studies have obtained empirical evidence of the minimum fish size and related tagging effects.
Since one of the attractive features of KNK10 is its relative stability against proteolytic degradation, we also investigated if hydrophobic tagging effected the peptide proteolytic stability.
In contrast, because an additional type-2 tag losses effect was not supported by the data, we cannot conclude there is a difference in continuous tag shedding and continuous mortality between the two types of tags.
We assessed tag-induced mortality by model-weighted average apparent survival estimates (total individuals, males and females were 0.733±0.086, 0.883±0.389 and 0.761±0.132, respectively) which are within expectations for minimal tag mortality effect.
This would agree with the fact that the plasma TAG lowering effect of fenofibrate was more marked at 14 weeks, when LV TAG content was reduced, than at 21 weeks, when it did not reduce LV TAG stores.
