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The plasmid presented an amino terminal export tag corresponding to the first 45 amino acids of rat somatostatin receptor 3 and a carboxy terminal HSV tag [39].
The addition of a tag corresponding to the first 19 amino acids of bovine rhodopsin at the amino terminus of the receptors allows assessment of surface expression with a tag-specific monoclonal antibody.
A tag corresponding to FAST-1 was identified in a recent study of the human antisense transcriptome (position 70840691 on the "-" strand of chromosome 9 in the HCT116 cell line) [14] (Papadopoulos, personal communication), which maps close to the start of intron 1 of the FXN gene (Fig. 3A), and further substantiates the existence of FAST-1.
The predefined order of dispensation of these nucleotides was determined according to the sequence tag corresponding to a hypervariable region of the Mycobacterium genome.
Whether these exons were expressed before the retroposition remains to be determined, but no expressed sequence tag corresponding to exons 3 and 4 alone could be identified in mammalian EST databases.
The deduced amino acid sequence of the full-length enzyme consists of 583 residues (including the N-terminal histidine tag) corresponding to a molecular mass of 65978.6 Da, which is in accordance with the apparent molecular mass estimated by SDS PAGE.
Similar(53)
An evolved strain, MITGM-173, gronn on a defined medium with 16 g L−1 glycerol, produced 2.3 g L−1 of TAGs, corresponding to 40.4% of the cell dry weight (CDW) and 0.144 g g−1 of TAG yield per glycerol consumed.
In a stationary phase occurring 3 days post-inoculation, the strain was able to completely utilize the sugar, and yielded 2.0 g/L for l-arabinose and 2.2 g/L for l-arabinose/d-glucose of TAGs, corresponding to 39.7% or 42.0%, respectively, of the cell dry weight.
We seriated 50 tags corresponding to each of the 10 expression profiles and 50 tags corresponding to noise.
Finally, Lib843 had 311,175 tags corresponding to 38,244 unique tags and the pooled library had 747,778 tags corresponding to 51,470 unique tags.
We selected 1,144,485 tags corresponding to 256,604 non-redundant tags, with lengths ranging from 17 to 25 nt, for further analysis.
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