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With a stringent criterion of 0 mismatches within the 16-nucleotide tag alignments, most of the tags aligned to the models but large numbers of tags did not.
Using a stringent criterion of 0 mismatches within the 16 nucleotide tag alignments, most of the tags aligned to the models but large numbers of tags did not.
The primers for these regions were developed from multiple species EST (Expressed Sequence Tag) alignments from P. tremuloides and P. trichocarpa[ 17].
However, in the process of ordering the DM reference sequences [ 18], we have also had a close look at sequence tag alignments of the complete RH BAC physical map to the DM reference chromosomes.
The WGP fingerprints were cleaned from chimeras by looking for BACs that gave false connections or friction alignments in preliminary versions of the physical map, and also by looking for BACs with chimeric WGP tag alignments to a pre-publication version of the Solanum tuberosum group phureja genome sequence [ 29].
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Statistical analysis of clean tag alignment was conducted, including analysis of total clean tags and distinct clean tags (Additional file 3: S3, Additional file 2: S2).
Statistical analysis of clean tag alignment was conducted, including the analysis of total clean tags and distinct clean tags (Additional files 2 and 3).
Observed p53-occupied sites in the PC12 genome were visualized by plotting normalized tag alignment locations relative to a hypothetical gene size of 100 kb.
These include the tag alignment parameters and the sequence of contaminating sequences, which will be highlighted in the tabular format (Supplementary Material).
The tag alignment position's upstream and downstream genomic sequences (200 bp each) were extracted from the genome assembly using homemade Perl scripts.
Given that a reference genome was not available, the Universal Network-Enabled Analysis Kit (UNEAK) nonreference pipeline (Lu et al., 2013) implemented in TASSEL version 3.0.160 (Glaubitz et al., 2014) was used for tag alignment and subsequent SNP calling.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com