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Rolink, A., Grawunder, U., Winkler, T.H., Karasuyama, H. & Melchers, F. IL-2 receptor α chain (CD25, TAC) expression defines a crucial stage in pre-B cell development.
TAC expression was mainly confined to the leaves and annual shoots, whereas LAZY was mainly expressed in the annual shoots and axillary buds.
Furthermore, scrutiny of TAC expression changes was performed with MapMan [ 55, 56].
Parallel experiments utilizing the variously transfected HeLa clone 1.E cells and stained for Tac expression recapitulate all these findings.
For constructing plasmid pBT'Tmcs (Tcr) the tac expression cassette and chloramphenicol (Cm) marker of pJT'Tmcs (Verhoef et al. 2010) were amplified by PCR using primers 1 and 2 (Table 2).
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Interestingly, in IKKalpha knockout MEFs, LMP1 hyperinduced MIP-2, TNFalpha, and I-TAC expression, consistent with a role for IKKalpha in down-modulating canonical IKKbeta activation or its effects.
Although an early diagnosis is not an easy task in routine clinical settings, considerable research has been done to incorporate early and robust biomarkers to the diagnosis, such as chemokines IP-10 and I-TAC expression in peripheral blood [ 120].
Expression constructs for mupM, tmlM and mupA (from S. aureus) were constructed based on tac promoter expression vector pJH10 [5].
Glandorf et al. (2001) modified the plant growth promoting P. putida strain WCS358r to produce the yellow pigment PCA (phenazine1- carboxylic acid) by P tac -controlled expression of the genome-integrated phzABCDEFG operon from P. fluorescens 2-79.
After TAC, myocardial expression of miR-143 and miR-145, measured by quantitative real time reverse polymerase chain reaction (qRT-PCR), did not change (data not shown); in contrast, expression of these miRs was dramatically reduced in the aorta upstream and downstream of the TAC site (Fig. 2a).
Consistent with the lack of effect of TAC on the expression of the α1-subunit, ouabain treatment also did not affect the expression of α1 protein in the control hearts (Fig. 8a).
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