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The deposition of Pd or Pt can enhance the oxidation resistance of TaC (1 0 0).
Meanwhile, the TaC (1 0 0) decorated by monolayer Pd still exhibited outstanding catalytic activity for O2 dissociation.
It is found that the adsorption sites of O2 are the Ta-Ta bridge sites on both the partially covered TaC (1 0 0) surfaces by Pd and Pt, M4/TaC (1 0 0) (M = Pd and Pt), while the 4-fold metal hollow sites and the metal-metal bridge sites are preferred on the fully covered TaC (1 0 0) surfaces by Pd and Pt monolayer, MML/TaC (1 0 0), respectively.
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A His6-tagged TAC-1 full length fusion protein was used to immunize mice.
A polyclonal antibody directed against full length TAC-1 recognized the endogenous protein in early embryos [54].
WUHC performed all fusions except for TAC-1 and SAX-7, which were performed by the SLU Hybridoma Facility.
(A ) Localization of TAC-1 GFP TAC-1 GFP 2 min after injury; TAC-1 with a C-terminal GFP tag relocalized similarly to N-terminally tagged TAC-1.
TAC-1 and ZYG-8 interacted by two-hybrid assay, and TAC-1 interacted strongly with ZYG-8ΔKD.
(C ) Strategy for neuron-specific deletion of tac-1 mutants with Mos-SCI single copy transgene of floxed tac-1.
After immunoprecipitation of EFA-6N150 we could detect both TAC-1 and ZYG-8, and the interactions between EFA-6 and TAC-1 (or ZYG-8) were not affected by the presence of ZYG-8 (or TAC-1).
Animals not expressing P ttx-3 -RFP, that is, tac-1 ok3305); tac-1 ok3305tac-1-lox Mos-SCI), were used as negative control.
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