Low statistical errors (see tables) validate the repeatability of the measurements and justify the representative outcome for our analysis.
Additional cross table analysis validated these results (Table 1).
The subset of 15 candidate reference genes (Table 2), validated on qRT-PCR, exhibited a broad range of expression levels from 17.93 to 36.66 cycle threshold (Ct) values (Table 3, Figure 4).
Therefore, the interpretation of the derived data was based upon certain theoretical predictions (Table 1) validated by simulations (above).
Requiem (single-peptide "hit"; Table 1), validated in this study as BAF-associated in vivo, is a relatively uncharacterized transcription factor that regulates apoptosis in the myeloid lineage [47], [48].
The thematic networks and the schematic table were validated through in-depth discussions between the researchers.
Additional file 4: Table summarizing validated discordantly mapped read-pairs in the Illumina HiSeq reference alignment of C. difficile 630 Δ erm (LUMC) versus 630.
The results of the error study (Table 2) validated the morphometric midsagittal plane and confirmed its accuracy and reliability previously reported [ 15, 18– 20].
The quantitative real-time RT-PCR (qRT-PCR) analysis of randomly selected genes (CG34040, CG64267, CG9468, CG11909, CG6524, CG17571, CG10374) using gene-specific primers (Additional file 1: Table S1) validated the RNA-Seq data (Additional file 1: Figure S2).
The MMTA task flows and task decomposition tables were validated by personnel from FHNC.
