Diffraction data were collected at beamline X29 at the NSLS (Table 2) and processed with XDS [34].
Quantitative real time Taqman gene expression analysis was performed according to the manufacturer's instructions using the probe sets listed in Table S2 and processed as described [63].
Diffraction data were collected at synchrotron sources (see Table 1), and processed with HKL2000 (Otwinowski and Minor, 1997) or XDS (Kabsch, 2010).
A single cell suspensions (100 μL) were prepared in triplicates (comprising 10,000 cells each) and incubated with mAbs (50 μL) (Table 1) and processed as described previously [ 29].
Nanostring staff designed capture probes for 50 genes from the Affy GeneChip (supplementary material Table S5) and processed 48 RNA samples.
A two-wavelength dataset was collected at the Diamond Light Source (Harwell, U.K.; Table 1) and processed using the CCP4 suite [ 23].
The balanced dataset consisted of 30,500 SNPs mapped to unique PDB structures (see Table 2) and processed without any errors, and a random selection of 30,500 PDs (mapped to unique PDBs).
A final set of 47,220 tags assigned to 11,238 BACs, representing only 48.5% of the expected single tags (47,220 vs 97,293) and 29.6% of the expected number of tags per BAC (17.3 vs 58.4), was obtained (Table 1) and processed.
Proteins were separated on precast Novex 12 or 4 12% polyacrylamide Bis Tris gels, transferred to PVDF membranes, probed with various antibodies (Supplementary Material, Table S1) and processed by ECL detection as previously described (60).
Slides were dried overnight on a heating table at 42°C and processed the following day.
