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There was a reduction in the strength of this pattern when the central TA step was changed to TG/CA, and a near loss of the periodicity when the TA flanking bases were exchanged from C and G to G and C (middle and bottom panels).
As an example of estimating T in this paper, we first performed simulations of the TA localization with a typical TA step of 553.5 m by using considered measurement sets.
The 10 bp sequences surrounding these TA step conferred high stability and positioning activity when they replaced the −10 to −20 bp region in Fragment 67 [37], [37].
Mutational analysis revealed that both the TA step and its flanking bases are required for high affinity octamer binding and translational positioning.
The rotational orientation of the TA step at the −15 site is important for the high nucleosome positioning activity and stability of Fragment 67 since reductions in these functions were seen when the TA step was translocated by as little as 1 bp in either direction [37].
Changes in the TA step in fragment 67 resulted in reductions in nucleosome stability and positioning activity and these changes were accompanied by corresponding reductions in FVOs (37, Table 1A).
Similar(44)
Taing stepped out to ask for help, but the car didn't stop; and in the glare of the headlights, Sa saw a black Lexus, an LX 470 SUV, with the windows open.
However, since TA steps are characterized by lowest stability [22] it remains open as yet whether such sequences with periodical potential kinks at TA may reside in natural nucleosomes as well.
As we agreed on a price and I was about to step into Ta's ample 30-foot turquoise junk, Mr. Dzung asked me if I had a money belt.
Since the TA base step is the most flexible of all base steps, it is most able to accommodate the stress created at the pressure points.
Why is the TA base step so flexible?
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