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The mean of E p/Ē t intensity ratios in SN for TC-specific probes was shifted toward TC E p/Ē t intensity ratios (Fig. 3C) suggesting higher level of gene expression in the A and B genomes combined.
Percentage of cells methylated at the nine CpG sites in the A vy IAP region was quantified by phosphor imaging (percentage of cells methylated = [100 × (C intensity)/(C intensity + T intensity)].
As expected, the AT-specific probes had greater E p/Ē t intensity ratio in AT than in TC and TC-specific probes had greater E p/Ē t ratio in TC than in AT (Fig. 3A and 4).
This is evident from the well-defined two-cluster pattern produced by plotting the E p/Ē t intensity ratios estimated for 40,281 probes in AT (y-axis) and TC (x-axis) lines (Fig. 3A).
The significant fraction of E p/Ē t intensity ratio values in SN for AT-specific probes falls between the intensity ratios obtained for AT and TC lines (Fig. 3B), consistent with previous observations that the gene expression in polyploids tends to stabilize at mid-parent level [ 9, 27].
Variables include expression summary statistics Total (T), Intensity (I), and Evenness (E), the rate of nonsynonymous (dN) and synonymous (dS) subsitutions per site, the ratio of dN/ dS after adjusting dS (dNdS_adj), the number of exons (No.Exons), the total protein-coding length in nucleotides (Protein Length), the total intron length (Intron Length) and the amino acid diversity (AA Diversity).
Similar(51)
At UV 350 nm and in Sun Test (Intensity: 750 W/cm2), sucralose was persistent to photolysis in both natural water matrices (SW and CW).
The compound also had no detectable effect on the final thioflavin-T intensity.
The thioflavin-T intensity decreases rapidly after addition of resveratrol, suggesting that the compound might interfere with thioflavin-T binding.
The increased thioflavin-T intensity allows the full curve to be monitored in the presence of a 20-fold excess of the compound.
A slower, additional, loss of signal occurs, but the thioflavin-T intensity does not return to baseline even after incubation for 3 days.
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