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The mean of E p/Ē t intensity ratios in SN for TC-specific probes was shifted toward TC E p/Ē t intensity ratios (Fig. 3C) suggesting higher level of gene expression in the A and B genomes combined.

Percentage of cells methylated at the nine CpG sites in the A vy IAP region was quantified by phosphor imaging (percentage of cells methylated = [100 × (C intensity)/(C intensity + T intensity)].

The E p/Ē t intensity ratios of PSFs having a perfect match with either AT or TC target sequences are expected to be higher than the intensity ratios of probes hybridizing with mismatching target sequences (Fig. 3B,C, and 4).

As expected, the AT-specific probes had greater E p/Ē t intensity ratio in AT than in TC and TC-specific probes had greater E p/Ē t ratio in TC than in AT (Fig. 3A and 4).

This is evident from the well-defined two-cluster pattern produced by plotting the E p/Ē t intensity ratios estimated for 40,281 probes in AT (y-axis) and TC (x-axis) lines (Fig. 3A).

The significant fraction of E p/Ē t intensity ratio values in SN for AT-specific probes falls between the intensity ratios obtained for AT and TC lines (Fig. 3B), consistent with previous observations that the gene expression in polyploids tends to stabilize at mid-parent level [ 9, 27].

Variables include expression summary statistics Total (T), Intensity (I), and Evenness (E), the rate of nonsynonymous (dN) and synonymous (dS) subsitutions per site, the ratio of dN/ dS after adjusting dS (dNdS_adj), the number of exons (No.Exons), the total protein-coding length in nucleotides (Protein Length), the total intron length (Intron Length) and the amino acid diversity (AA Diversity).

Among the TC-specific probes 4,124, showed statistically significant departure of E p/Ē t intensity ratios from the expected mid-parent values with 3,718 probes (90.2%) showing the increase of TC parent contribution to SN expression whereas 406 probes (9.8%) demonstrated the decrease of TC proportion in total gene expression.

At UV 350 nm and in Sun Test (Intensity: 750 W/cm2), sucralose was persistent to photolysis in both natural water matrices (SW and CW).

The compound also had no detectable effect on the final thioflavin-T intensity.

The thioflavin-T intensity decreases rapidly after addition of resveratrol, suggesting that the compound might interfere with thioflavin-T binding.

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