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From the synthetic systems, we generated 192 training and 192 validation data sets under different omega interval conditions.
To achieve direct, continuous risk assessment of air pollution using biological systems, we generated transgenic sensor mice that produce secreted alkaline phosphatase (SEAP) under the control of AhR.
Using linkage-specific assembly systems, we generated Lys63-, Lys48-, Lys11- and Met1-polyubiquitinated model substrates in vitro, (see Experimental Procedures), which were treated with a panel of DUBs.
To determine the functions of the identified putative protein secretion systems, we generated derivatives of strain 3841 carrying insertion mutations in prsD (A895), toaD (A913), tobD (A896), virB6 (autA), A1010(autB0), A1011(autC1), A1012(and12) and tatC (A1052).
In order to develop technology for sequential mutagenesis in vivo using two site-specific recombinase systems, we generated Col1a1 FRT-Cre-ER-T2-FRT and Col1a1 FRT-STOP-FRT-Cre-ER-T2 mice.
For each of the 15 pairwise combinations of the six physiological systems, we generated all possible biomarker groupings in two groups of equal size to the original two groups.
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In our system, we generated the vortex by the circular motion of an end effector.
To demonstrate the utility of this system, we generated stable transgenic lines which allow inducible tissue-specific expression of activated K-ras V12). K-ras V12
To conceive the scoring system, we generated a receiver operating characteristic (ROC) curve for each biological value and calculated the area under the ROC curve (AUROCC) with its 95% confidence interval.
To demonstrate the efficacy of this system we generated FAdV-9 ORF2, and FAdV-9 ORF11 deleted, CAT marked and unmarked FAdV-9 infectious clones (FAdmids), and replaced either ORF2 or ORF11, with an EGFP expression cassette or replaced ORF2 with an EGFP coding sequence via the unique SwaI sites, in approximately one month.
To directly test whether the protective effects of PI3Kγ are due to its function in the immune system, we generated bone marrow chimeras.
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