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Several relevant levels are in play in this research: molecules, transport systems in cells, cells, tissues and organism.
These two proteins constitute one of two major redox regulatory systems in cells (together with the glutathione system) [31].
The thioredoxin (Txn) dependent system, comprising of cytosolic (Txn1) and mitochondrial (Txn2) thioredoxins, various Txn dependent peroxidases (peroxiredoxins) and the selenoenzymes thioredoxin reductases 1 3, is one of the major redox systems in cells involved in the control of cellular redox balance [2], [8].
Gene regulatory networks (GRNs) are fundamental for sustaining complex biological systems in cells.
With this function being protection against glucose-derived H2O2, several reflections can be made regarding the roles of selenium and parallel antioxidant systems in cells.
In addition, the status of the major thiol redox systems in cells during aging and its modulation by the WRN protein has hardly been explored.
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In this review we discuss new microfluidic devices for gradient generation and applications of those systems in cell analysis.
CXCL-1 (DY453, R&D Systems), CCL-2 (900-K126, PeproTech), and TNF-α (DY410, R&D Systems) in cell-culture supernatants were quantified by ELISA according to the manufacturers' instructions.
We have applied this approach to amino acid metabolism, one of the systems in cell-free biology that limits protein synthesis reactions.
Physio-biochemical analysis confirmed the direct effect of fatty acids composition, lipoxygenase activity and antioxidant systems in cell responses under CS.
This is a highly efficient tool for the rapid generation of stringently controlled Tet-inducible systems in cell lines.
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