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The classification systems for this study were selected because they are the most commonly used and studied for fractures of the proximal radius [ 14].
We have used seven different systems for this study (Additional file 1); the complete 3D model structure of the complex (Her2-P-T), the receptor bound to trastuzumab (Her2-T) and pertuzumab (Her2-P), the apo-receptor (Her2), the two independent antibodies (pert and trast) and both antibodies co-localized as they are found in the complex (Abs).
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The simulated system for this study contains four silver nanoparticles, and the corresponding geometries are shown schematically in Fig. 1a.
Where the previous comparable studies used an older two-pod camera system, for this study, the fourth-generation 3dMDface imaging system was used.
The community of Grand Marais uses one of the few operating sewage lagoon/constructed wetland treatment systems in the province and was selected as a model system for this study.
We found that CHO cells were a good system for this study as APC expressed well in these cells and allowed us to follow the localization of the protein.
We used a coupled chamber– in vitro exposure system for this study (Sexton et al. 2004).
Given the structural characterization and amenability of the system to extensive mutagenesis and kinetic analysis, AP is a perfect model system for this study.
The light-driven proton pump bacterioRhodopsin (bR) was chosen as a model system for this study because it represents one of the most extensively studied transmembrane proteins.
And finally, thanks to a multitude of studies, its internalization and trafficking properties are well known and thus provide an optimal validation system for this study.
As a model protein system for this study, we chose an important enzyme involved in glycolysis and gluconeogenesis, phosphoglucose isomerase (PGI; EC 5.3.1.9).
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