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We are coordinating sample collections in-between TMU and LUH, utilizing robotic -80 °C sample handling systems as previously described [1],[1]]–[1]].
The chromatographic separation was optimized by means of computer-assisted method development by use of a special chemometric tool developed specifically for LC MS systems, as previously reported by the authors.
Forty-eight hours later, luciferase activities were determined using luciferase enzyme assay systems as previously described [17].
Endogenous IL-16 was neutralized using 1 µg/ml anti-IL-16 mAbs (R&D Systems) as previously described [6].
Lung explants isolated from E12.5 WT and CKO embryos were cultured and treated with 200 ng/ml FGF9 (R&D systems) as previously described [8].
In brief, 293T cells were transfected with pLKO.1-derived constructs together with the pHR'8.2ΔR and pCMV-VSV-G packaging systems as previously described [63].
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Calcium imaging was performed on free-moving worms by the imaging system as previously described (Li et al. 2012).
The antioxidant activity was also determined in terms of measurement of percent inhibition of peroxidation in linoleic acid system, as previously reported [35].
The samples were subjected to a precolumn derivatization in the needle of the autosampler with OPA-3-MPA, and injected in the HPLC system as previously reported [28].
The ammonium concentration was determined during fermentation using a continuous- flow analysis system as previously described (Mendes- Ferreira et al. [2004]).
Note that this wavelength interval was decided according to the range of the hemispherical radiative property measurement system as previously indicated.
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