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The installed support systems as per empirical methods were cross-checked by means of numerical modeling, field observations and engineering judgments.
Fig. 2 Distribution of housing systems as per types of structures in Nepal Fig. 3 Distribution of houses as per types of wall in Nepal Fig. 4 Distribution of houses as per types of roof in Nepal.
Eotaxin 3 and IP-10 levels were measured in supernatants of cultured epithelial cells in the ALI as well as the submerged conditions using specific DuoSet ELISA kits (R&D Systems) as per manufacturer's instructions.
Supernatants were collected and 100 µl was used in an IL-10 ELISA (R&D Systems) as per manufacturer protocol.
7 µm sections were cut and fixed with ice cold 1∶1 acetone/methanol before staining using a DAB- immunohistochemistry detection kit (R&D systems), as per the manufactures instructions.
Sections were again washed in PBST and the biotinylated secondary antibody detected using LSAB®2 Streptavidin-HRP (Dako, Glostrup, Denmark) and diaminobenzidine (DAB) (Dako) systems as per manufacturer's instructions and finally counterstained with haematoxylin.
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Over the weekend, the BKK chairman took to the radio in Hungary to blame T-Systems for the situation and T-Systems, as per the usual consulting crisis playbook, released a rambling non-apology.
The blots were then developed with an ECL detection system as per the manufacturer's instructions.
We can select appropriate distributed system architecture and suitable heterogeneous protocol for building collaborative system as per requirements.
Hybridisation probe (521-bp chimeric gene) was labeled non-radioactively using Gene Images AlkPhos Direct Labeling and Detection System as per manufacturer's instructions (Amersham Biosciences, Hongkong).
Sensitivity of the steady state behavior of such a two-phase natural circulation system to different system parameters has many implications vis-à-vis performance of the system as per the design intent under various operating conditions.
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