They leverage our knowledge of metabolism to test these hypotheses using cell culture systems and mouse models.
The following antibodies were used: rabbit polyclonal anti-hPGRN (1∶1000; Alexis Biochemicals); goat polyclonal anti-hPGRN (1∶1000; R&D Systems) and mouse monoclonal anti-actin (1∶5000; Sigma).
In line with our findings, over-expression of TARBP2 enhances cell proliferation and transformation in both cell culture systems and mouse models.
The following primary antibodies were used: mouse monoclonal biotinylated anti-CD49f (BioLegend), mouse monoclonal anti-Dazl (AbD Serotec), goat polyclonal anti-VASA (R&D Systems), rat monoclonal anti-STELLA (R&D Systems), and mouse monoclonal anti-VIMENTIN (Dako).
Mouse monoclonal anti-human VEGFR1 antibody (IgG1, #MAB321, R&D Systems), goat polyclonal anti-human VEGFR2 antibody (IgG, #AF357, R&D Systems) and mouse monoclonal anti-human VEGFR-3 antibody (IgG1, #MAB3491, R&D Systems).
Puncta were immunostained with guinea pig anti-VGAT (1 500; ref. 131 004, Synaptic Systems) and mouse anti-γ2 (1 500; ref. 224011; Synaptic Systems) and NG2 cells with rabbit anti-NG2 (1 400; ref AB5320, Millipore).
A large body of work in human cell culture systems and mouse models has documented the biological and genomic consequences of telomere attrition and how these consequences relate to the development of premature aging and cancer [ 4].
For GFAP-vimentin colocalization studies, the epitope retrieval step was not performed and following primary antibodies were used: rabbit anti-vimentin (1 200, Synaptic Systems) and mouse anti-GFAP (1 200, Sigma).
He was the first to find an equivalant in humans to the correlation between the H2 system and mouse leukemia.
After blocking with 5% milk powder (Carl Roth GmbH) for 1 hr at room temperature, membranes were incubated with rabbit anti-CpxI/II (1 1000; Synaptic System) and mouse anti-tubulinIII (1 750; Sigma Aldrich) antibodies overnight at 4°C.
