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Production of these biologics with various plant systems and expression strategies is examined.
E. coli expression of Ab derived fragments has been highly successful and a number of vector systems and expression strategies exist ([ 21] and references herein).
However, most CSCs appear to evade cytotoxic therapies or irradiation through active mechanisms, such as ATP-binding cassette (ABC) transporter proteins overexpression, active DNA repair systems, and expression of anti-apoptotic factors [ 4, 8].
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It is expressed at greatest levels in the central nervous system, and expression is localized to a distinct area called the nucleus incertus (NI), situated in the caudoventral region of the pontine periventricular gray (6, 48).
Human MutS homolog 2 (MSH2), a crucial element of the highly conserved DNA mismatch repair system, and expression of MSH2 have been down-regulated by Hsp90 function inhibition in human lung cancer.
Quantitative reverse transcriptase PCR (RT-PCR) was performed using validated TaqMan Assays (Applied Biosystems) on an Applied Biosystems 7900HT PCR system and expression normalized relative to GAPDH as described [23].
Aquaporins are water selective channels with possible roles in the nervous system and expression levels were upregulated after brain injury.
In mice, L. paracasei F19 has been shown to affect the immune system and expression of genes involved in energy homeostasis and insulin sensitivity, and to regulate body fat storage and lipoprotein metabolism.
Toll-like receptors (TLRs) are important players of the innate immune system, and expression of the 10 TLRs known in humans depends on the cell subset and differentiation status.
Most of the studies reporting cannabinoid receptors coupling to specific kinases were performed in heterologous expression systems and the expression level of these receptors both surpass those reached by endogenously-expressed receptors and favor promiscuous coupling to effector proteins.
Here we briefly describe these systems and the expression data upon which our fits are based.
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