Sentence examples for systematic detergent from inspiring English sources

This must be accompanied by systematic detergent screens to select detergents that are suitable for long-term stabilization of functional membrane proteins.

After systematic detergent screening, the zwitterionic detergent fos-choline-14 (FC14) was found to be most effective for solubilization and was subsequently used throughout the entire solubilization and purification.

To date, a systematic detergent screen has not been described for the reconstitution of small helical membrane protein samples into lipid environments for solid state NMR.

However, these artificial lipid-mimicking molecules may influence the binding of hydrophobic ligands, as observed in the case of a steroid binding site in cytochrome c oxidase where a systematic analysis of detergent effects led to a clearer understanding of competitive behavior between ligands and detergents and a more precise definition of the binding domain.

After a systematic screen of 96 detergents, the zwitterionic detergents of the Fos-choline series (FC9-FC16) emerged as the most effective for isolation of the hCRs.

We again carried out a systematic search for optimal detergents Each Trx-hCR expressed by pBAD-hCRs in Top10 was simultaneously solubilized in each of the 96 detergents in a 96-well plate and the extract was examined using Dot blot detection.

Therefore, a systematic approach is required to select the optimal detergents to achieve solubilization and stabilization of each target protein.

Therefore, a systematic approach for screening a wide range of detergents is essential for obtaining the expressed hCRs.

These results shows the great potential of GRecon for the 2D crystallization of membrane proteins, which requires systematic variation of parameters such as lipids and detergents, protein concentration, and lipid/protein ratio.

(family Plantaginaceae) were collected from the healthy plants grown in an experimental garden of our college and were washed with 2.5% (w/v) systematic fungicide (Bavistin) for 15 min followed by 2.5% liquid detergent (Tween-20) solution for 2 min.

To date, a systematic method by which to evaluate the effect of the solubilizing detergent on proteoliposome preparations for solid state NMR of membrane proteins has not been presented in the literature.