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The results are given in Additional File 2. Genomes were searched by systematic BLAST comparisons to proteins from A. thaliana and Saccharomyces cerevisiae.
During the reconstruction process, systematic Blast and interrogation of metabolic databases were performed to refine weak - or missing - genome annotation information or new published data.
Searching EMBL EST Fungi cDNA libraries (http://www.ebi.ac.uk/Tools/sss/wublast/nucleotide.html) yielded further snoRNAs from Basidiomycota, which were not found in the systematic blast searches.
In order to identify proteins which are chlamydiae-specific, systematic BLAST searches were carried out on different proteins in the genomes of Chl.
To determine whether the existing set of mouse Psg genes was complete we performed systematic BLAST searches of a variety of public DNA sequence databases.
Sequences of all known mammalian GLUT family members were subject to systematic BLAST searches against the completed genomes of bacteria, fungi, protists, plants, invertebrates and vertebrates (Table 1).
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the systematic blasting.
Consistent with this conclusion, systematic blast-search analyses with individual DBD exons failed to retrieve proteins other than COE proteins from databanks.
Our systematic blast-search for coe orthologs in DNA sequence databanks confirmed that coe genes are metazoan genes present at a single copy per genome, except for vertebrates.
In this study, several novel members of helicase family were identified by a systematic BLAST-based homology search, including two plant helicase-like genes of F52G3.3 and F52G3.4 and five C. elegans-specific SNF2-like genes.
Our systematic blast-search for coe (collier/olf-1/ebf) orthologs in DNA sequence databanks confirmed that coe genes are metazoan genes present at a single copy per genome, except for vertebrates.
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