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The sensitivity of Zp to systematic alignment errors can be exploited to identify regions of potential misalignment.
We show that current non-parametric covariation statistics are sensitive to sequence misalignments and that this sensitivity can be used to identify systematic alignment errors.
In the spirit of classifying systematic alignment errors introduced by [48], we refer to this problem as gap dribble, since short alignments are dribbled along the large gap.
Furthermore, we show that new statistics
Systematic alignment errors may be introduced by reads originating from repetitive regions.
An alternative strategy could be a more systematic alignment of columns with the same ocularity, as suggested by the stripy pattern in macaque monkeys.
Similar(54)
To eliminate these systematic alignments and variations of size, we also employed a 3D constrained condition, in which the spheres were distributed in 3D space such that their centers were constrained to lie on the depicted object surface at randomly selected positions.
To eliminate these systematic alignments and variations of size, we also employed a 3D constrained condition, in which the spheres were distributed in 3D space such that their centers were constrained to lie on the depicted surface at randomly selected positions.
We show that current criteria are insufficient to build alignments for use with covariation analyses as systematic sequence alignment errors are present even in hand-curated structure-based alignment datasets like those from the Conserved Domain Database.
The camera sensor was aligned to the stage so that the angle between their axis was less than 0.01 degrees, thus avoiding systematic image alignment errors.
These numbers suggest false-positive rates of 15%and4%4% respectively, mainly due to transcriptome assembly problems, SNPs, somatic mutations and systematic mis-alignments.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com