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The DEPP interaction network was build automatically by the STRING Search Tooll for the Retrieval of Interacting Genes/Proteins) system with default setting except that organism, confidence score), and additional (white) nodes were set to "human", "0.20", and "10", respectively[50], [51].
However, protective lung ventilation is standard practice throughout our medical system with default ventilator orders for pressure-limited, low tidal volume parameters, so significant non-compliance was unlikely.
Quantitative real time PCRs (qRT-PCRs) were prepared using the SYBR Green Master Mix kit (Applied Biosystems) and run in an Applied Biosystems 7500 Fast Real-Time PCR system with default parameters.
For each indel in our set the sequence of its encompassing protein was blasted against the PDB using blastp from the NCBI QBlast system with default parameters to obtain information on the secondary structure of the protein.
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Apple's latest mobile operating system comes with "default encryption" and Google's Android is about to follow suit.
The threshold cycle (Ct) was automatically determined for each reaction by the system set with default parameters.
The threshold cycle (CT) was automatically determined for each reaction by the system set with default parameters.
The Ct was automatically determined for each reaction by the Applied Biosystems 7500 Real-Time PCR System set with default parameters.
Quantitative real time PCR reactions were prepared using the SYBR Green Master Mix kit (Applied Biosystems) and performed in an Applied Biosystems 7500 Fast Real-Time PCR system (Applied Biosystems) with default parameters.
In summary, reactions were prepared using the SYBR Green Master Mix kit (Applied Biosystems) and performed in an Applied Biosystems 7500 Fast Real-Time PCR system (Applied Biosystems) with default parameters.
The Ct was automatically determined for each reaction by the Light Cycler LC480 Real-time PCR system set with default parameters.
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