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Taking advantage of the multiplexable genome engineering feature of the CRISPR/Cas9 system, we sought to generate different kinds of immunodeficient mouse strains by embryo co-microinjection of Cas9 mRNA and multiple sgRNAs targeting mouse B2m, Il2rg, Prf1, Prkdc, and Rag1.
Using this system, we sought to understand the impact on tumor-specific T-cell responses of simultaneously removing a major brake on expansion via blockade of the co-inhibitory receptor CTLA-4, while at the same time actively driving proliferation and survival through activation of the co-stimulatory receptor 4-1BB.
To better understand the roles of LARK in the circadian system, we sought to identify RNA molecules associated with it, in vivo, using a three-part strategy to (1) capture RNA ligands by immunoprecipitation, (2) visualize the captured RNAs using whole-genome microarrays, and (3) identify functionally relevant targets through genetic screens.
As Spg and Mbc are the two DOCK family members predicted to be specific for Rac and mutations in either one exhibit defects in the nervous system, we sought to examine if embryos mutant for both mbc and spg resulted in enhanced nervous system defects.
Focusing on the German health care system, we sought to explore the attitudes of health professionals regarding their understanding of palliative care for older patients and its implementation.
In tandem with further research into this system, we sought to pursue potential routes to materials with enhanced oxidative stability as compared to the observed oxidation onset potential of 1.7 V (vs. Mg on Pt) for Mg(BH4 2.
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As we work to identify principles regulating the host:environment interactions that modulate the immune system, we seek to deepen our understanding of how this may impact the development of immunity to infection and efficacy of vaccination.
But for each system we seek to change, there are people in that system now people with more wisdom and answers than philanthropy has or gives people credit for.
In this system, we seek to optimize PAGE for a protein ladder spanning a mass range of 6.5 116 kDa.
Models need not (in fact should not) incorporate everything that we know about the biological system: we seek to understand the important components of reality, not to replicate the reality we do not understand.
In case of health problems in the first week after delivery, the majority (55%) of the women who delivered with a TBA made use of the formal health system: " we seek [professional] examination [tunafuata vipimo]".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com