Unlike lysate based cell free systems, the PURE system is reconstituted with individually purified factors essential for transcriptional and translational processes.
The unmeasured load torque and other disturbances in the PMSM system are reconstituted by the NFTSM observer and to be compensated during the controller design.
Purified proteins of the MinC/D/E system were reconstituted in membrane-clad soft-polymer compartments and imaged by confocal microscopy.
Studies conducted in the HIS mouse model indicate that the human immune system is reconstituted more efficiently in neonates compared with adult mice (Ishikawa et al., 2005; Ito et al., 2002).
In experiments with adult mice whose hematopoietic system is reconstituted with fetal liver cells from a miR-17/92Δneo/Δneo miR-17/92Δneo/Δneohembryoer of circulating lymphocytE14.5irculatheg B cells, splenumbercells and pre-B-cells bofe marrow circulatinggnificantlymphocytescirculatingth mice with reconstituted fetal liver cells from wild-type emBryos at 8–10 weeks post transplant.
Recombinant human WNT4 (cat. no. 6076-WN; R&D Systems) was reconstituted in sterile Phosphate-buffered solution (PBS).
Recombinant human interleukin-11 (rhIL-11, R&D systems) was reconstituted following user manual at a concentration of 50 μg/ml.
Intravitreal injections were performed as previously described in Joly et al. Rat recombinant TNF (R&D Systems) was reconstituted in sterile PBS containing 0.1% BSA (Sigma Aldrich) and injected at a concentration of 10 μg/ml.
Human recombinant OPG (500 ng/ml; amino acids 21 194 fused at the N-terminus to the Fc domain of human IgG1, ab998, Abcam) was reconstituted in PBS, aliquoted and stored at -80°C, and goat anti-human anti-MCSF blocking antibody (5 μg/ml, AB-216-NA, R&D systems) was reconstituted in PBS, aliquoted and stored at -20°C.
Nonetheless, in order for this paper to have the proper impact in the field and provide as useful a tool as possible for future researchers, we think that it is important to provide as much characterization as you can in the text of what your system is reconstituting in vitro.
To simplify the modeling of the electron transfer chain of the cytochrome P450cam system, it was reconstituted in solution as was reported in [18].
