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First, the solvated system was minimized by 500 steps of steepest descent minimization followed by 500 steps of conjugate gradient minimization with all protein ligand atoms in the system being restrained by 500 kcal/mol/Å in Cartesian space using harmonic potential.
After minimization of the solvent, the entire system was minimized by steepest descent for 1000 steps and then minimized by conjugate gradient descent for 1500 steps, which were otherwise identical to those of the first minimization routine.
After the MD simulation, the resulting periodic boundary system was minimized again to eliminate small geometry distortions using the Desmond [51 53] minimizer and finally also using the MacroModel minimizer (because of tighter convergence criteria).
Next, the energy of the whole system was minimized by removing the restraints in place and 10 000 steps of steepest descent minimization followed by 15 000 steps of conjugate gradient minimization.
The inherent noise of the extracellular recording system was minimized by using an independent battery supply.
Then, each system was minimized through 1000 steps, and MD runs were carried out for 100 ns at 310 K.
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Therefore, the optical system is minimized in volume and mass, and its manufacturing process is easier.
At the equilibrium state, the free energy of the system is minimized.
The final crystal shape is determined in such a way that the total free energy of the system is minimized.
We use the relaxed unit cell and adjust the lattice parameter such that total energy of the system is minimized.
To this end, the total transmitted power of the radar system is minimized to improve its LPI performance.
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