Exact(5)
The coupled system vectors contain both physical and modal components.
Lentiviruses were packaged in 293T cells ATCC-CRL-3216 ATCC-CRL-3216 ATCC-CRL-3216n packaging system vectors, pMDLg/pRRE, pRSV.Rev, pMDG VSVG.
The structures of system vectors and matrices for the dynamic model of the system under consideration can be deduced using the following differential equations: left( {P_{text{m}} - P} right)left( {frac{1}{s}M + D} right) = omega (1).
Thus, it appears that the additional sequences introduced by the loxP site in the original Clontech Creator system vectors may reduce expression levels.
Extended Experimental Procedures Cloning and Protein Preparation C. elegans SAS-6 Proteins DNA encoding full-length or fragments of C. elegans SAS-6 (Uniprot ID O62479) were cloned in pET system vectors (Novagen) encoding for N-terminal His6-tags or pGEX system vectors (GE healthcare) encoding for N-terminal GST-tags.
Similar(55)
By augmenting the system vector, a stochastic augmented system is obtained.
Sections were stained with antibodies using the ABC elite system (Vector).
Blocking and staining for cytokeratin was done using the MOM system (Vector; Burlingame, CA).
Bacteriomatch™ two-hybrid system vector kit and Bacteriomatch™ Reporter strain competent cells were purchased from Stratagene, USA.
Visualization of the c-Met or HGF on the sections was done using an immunoperoxidase system (Vector labs, Burlingame, CA) as per manufacturer's instructions.
For immunohistochemistry, slides were subjected to antigen retrieval in boiling in 10 mM NaCitrate; p63 antibody (#MS1081, LabVision) was used at 1∶400 with the Immpress detection system (Vector).
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